임플랜트에 연결한 영구자석의 자력이 뼈의 세포외 기질 생성에 미치는 영향에 관한 실험실적 연구
THE EFFECT OF PERMANENT MAGNET CONNECTING WITH DENTAL IMPLANT ON BONE EXTRACELLULAR MATRIX FORMATION
원인재, 이성복, 백진, 권긍록,
소속 상세정보
원인재 ( Won In-Jae ) - 경희대학교 치과대학 치과보철학교실
이성복 ( Lee Sung-Bok ) - 경희대학교 치과대학 치과보철학교실
백진 ( Baik Jin ) - 경희대학교 치과대학 치과보철학교실
권긍록 ( Kwon Kung-Rock ) - 경희대학교 치과대학 치과보철학교실
KMID : 0362420060440050574
Abstract
Statement of problem: The use of permanent magnetics is increasing in implant dentistry.
Purpose: This study is to know the effect of permanent magnetics on bone matrix formation of osteoblasts.
Materials and methods: The konus abutment-shaped permanent magnetics were connected to the implant fixture, and placed on the culture plate. The osteoblast-like cell Mc3T3E1 were used for cell culture. As the control group, the implants were connected to titanium healing caps, and cultured in the same conditions of experimental group. After 3. 7, 14 days, cells were cultured, and we measured and compared the amount of collagen type I, osteocalcin, which is bone matrix protein by Western immunoblotting analysis.
Results: As a result of Western immunoblotting analysis for estimating the amount of bone extracellular matrix, there was no difference between osteoblast of the experimental group and the control group during 3 and 7day-osteoblast culturing. However when cells were cultured for 14days, the amount of bone extracellular matrix was increased, on the experimental group.
Conclusion: From these results, magnetic field of permanent magnetics might have effect on bone formation of osteoblast, especially at initial stage of implant placement. Therefore, their clinical application for implant or bone graft could be possible.
키워드
Bone extracellular matrix;Permanent magnet;Collagen type I;Osteocalcin
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